Species-specific dissociation into single cells of live sea urchin embryos by fab against membrane components of Paracentrotus lividus and Arbacia lixula☆
[摘要] ThespeciesandstagespecificitiesofmembranecomponentsactiveinpromotingreaggregationofcellsdissociatedfromembryosofthetwoMediterraneanseaurchinspeciesParacentrotuslividusandArbacialixulahavebeenexamined.Membraneproteinsextractedwithbutanoleitherfrompurifiedmembranesorfromdissociatedcellswithoutsignificantreductionofviabilitypromotedreaggregationofboththehomologousandheterologousspecies.Extractsfrompluteiandblastulaewereequallyeffectiveinpromotingreaggregationofblastulacells.Bycontrast,Fab'spreparedfromIgGraisedagainsttheseextractsorpurifiedmembranesarestrictlyspeciesspecificbecausetheypreventreaggregationofcellsandactivelydissociateliveembryosofonlythehomologousspecies.NocorrespondingstagespecificityoftheFabwasobserved:Fabagainstextractsfromblastulaembryosalsocauseddissociationofplutei.AntigenicanalysisoftheextractsbytheOuchterlonytestrevealedthepresenceofcomponentsspecificforeachspeciesaswellasotherscommontoboth.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
