Postfertilization growth of microvilli in the sea urchin egg: New views from eggs that have been quick-frozen, freeze-fractured, and deeply etched☆
[摘要] Wehaveusedquick-freezing,freeze-fracture,anddeepetchingtostudypostfertilizationgrowthofmicrovilliintheseaurchin,Strongylocentrotuspurpuratus.Between1and2minpostinsemination,weobservedrapidgrowthofnumerousfinger-likemicrovillitoabout0.7μminlength.Subsequently,groupsoftwotofourmicrovillibecameraisedofftheeggsurfaceonbroadcytoplasmicbases.Finally,by5minpostinsemination,thesebaseshadbecomeinterconnectedbythinsheetsofcytoplasmtoformacontinuousnetworkofrufflesacrosstheeggsurface.Correspondingthinsectionsofquick-frozenandfreeze-substitutedeggsshowedthatthesemicrovillicontainednetworksofmicrofilaments.Thefinger-likeprocesseshadbundlesofparallelmicrofilamentsthatrantheirentirelength,whiletherufflesofcytoplasmcontainedsheetsofcrisscrossedmicrofilaments.Theseobservationssupportthehypothesisthatmicrovillargrowthresultsfromformationofnewmicrofilamentnetworks.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
