Oriented extracellular channels and axonal guidance in the embryonic chick retina☆
[摘要] Transmissionelectronmicroscopyandserialreconstructionoflumsectionswereusedtodeterminewhetheralignedextracellularchannelsprecedetheoutgrowthofopticfibersintheembryonicchickretina.Atstage16,justpriortothemigrationofopticaxonstowardtheopticstalk,extracellularspacesboundedbyneuroepithelialcellprocesses,inthesuperficial(vitread)regionoftheretina,werealignedtowardtheopticstalk.Theopticaxonssubsequentlyenteredandgrewwithinthesespaces.Afterformationoftheganglioncellfiberlayer(GCFL),thegrowthconesofnewopticaxonsenteredthemostvitreadportionofthatlayer.Hypertonicfixativescausedshrinkageofcellprocesses,resultinginintercellularseparation.However,growthconefilopodiaretainedclosecontactswithneighboringglialcellendfeetandwithopticaxonsinthesesolutions.Thissuggeststhatgrowthconesmaybeadherenttothesestructures.Transmissionelectronmicroscopyinconjunctionwiththeuseofhypertonicsolutionsmaybecomeausefultechniqueforassayingintercellularadhesivity.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
