Reevaluation of cell surface protein release at fertilization and its role in regulation of sea urchin egg protein synthesis☆
[摘要] Wehavereexaminedconditionsforreleaseoflabeledcellsurfaceproteinsfromseaurchineggsandtheeffectsofexogenouscellsurfaceproteinonthestatusofproteinsynthesisinactivatedeggs.Asreportedearlier(J.D.JohnsonandD.Epel,1975,Proc.Nat.Acad.Sci.USA72,4474–4478),wefindinseminationandtheCa-ionophoreA23187inducereleaseoflabeledcellsurfacepolypeptidesbutincontrasttothatreportwedonotobserveincreasedreleaseduringincubationinammoniaornicotine.Electrophoreticanalysisofthe125I-labeledpolypeptidesreleasedafterinseminationindicatesthatthecellsurfaceproteinfractioncontainsaminimumof10polypeptidesrangingfrom22,000to200,000daltons.Infiveexperiments,thecellsurfaceproteinsreleasedduringtheammoniaincubationhadnosignificanteffectonthestatusofproteinsynthesisinpartiallyactivatedeggs.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
