Ligatin from embryonic chick neural retina inhibits retinal cell adhesion☆
[摘要] Ligatin,afilamentouscell-surfaceproteinpurifiedfromembryonicchickneuralretina,hasbeenfoundtoinhibitthereassociationofdissociatedretinalcells.Thisinhibitionwasdemonstratedusingtwomethods,asinglecelldisappearanceassayandanimprovedmonolayercollectionassayutilizingmicrotiterplates.Monomericligatinatapproximately20μg/mlinhibitedratesofadhesion,butpolymericligatinandtrypticfragmentsofligatinwereineffective.Ligatin'sinhibitoryeffectissuggestedtobemediatedthroughbindingtoretinalcellsurfacessincepreincubationofdissociatedretinalcellswithmonomericligatininhibitedthecells'adhesivenessandremovedtheinhibitoryactivityfromtheculturemedia.Ligatinhomologuespreparedfrommammaliantissueswereineffectiveininhibitingretinalcelladhesion,suggestingatissueand/orspeciesspecificity.SimilaritiesinphysicochemicalandbiologicalpropertiessuggestthatligatinmaybetheinhibitorofadhesionpreviouslydescribedbyMerrelletal.[Merrell,R.,Gottlieb,D.I.,andGlaser,L.(1975).J.Biol.Chem.,250,4825].
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
