A cytoplasmic cAMP-binding protein in Dictyostelium discoideum☆
[摘要] AcytoplasmiccAMP-bindingproteinfromDictyosteliumdiscoideumwaspurifiedabout1200-fold.ThebindingproteinisrelativelyspecificforcAMP,butalsobindssomeotheradeninederivatives;ithasamolecularweightofapproximately185,000andanapparentKDof1μMcAMP.ThehighestlevelofcytoplasmiccAMP-bindingactivityisfoundinamoebaewhichhavebeenstarvedfor0–2hr.AmoebalextractscontaininhibitorsofcAMPbindingwhichareremovedbychromatographythroughSephacrylS200.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学(综合)
[关键词] [时效性]