Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites☆
[摘要] Thespecificityofadhesionbetweenembryonalcarcinomacellsandfibroblasticcellsofvariousoriginswasstudied.EmbryonalcarcinomacellshaveintercellularadhesionsitesrequiringCa2+(CDS).ThesesiteswerefoundtobesensitivetoproteasesbutresistanttotheminthepresenceofCa2+.CDSwithasimilarproteasesensitivityispresentinfibroblasticcells.Whenembryonalcarcinomacellsofdifferentlinesweremixed,theyadheredtoeachothernonselectivelybyCDS.NonselectiveadhesionbyCDSoccurredalsobetweenfibroblasticcellsofvariouslines.Whenembryonalcarcinomaandfibroblasticcellsweremixed,theypreferentiallyadheredtohomotypiccells.FabfragmentsofantibodiesraisedagainstF9cells(anullipotentlineofembryonalcarcinoma)inhibitedtheadhesionbetweenembryonalcarcinomacellsbutnotbetweenfibroblasticcells.ThisinhibitoryactivityofFabwasabsorbedwithembryonalcarcinomacellswithCDS,butnotwithfibroblasticcellswithCDSorembryonalcarcinomacellsfromwhichCDSwasexperimentallyremoved.SDS-polyacrylamidegelelectrophoresisofradioiodinatedcellsurfaceproteinsshowedthatthepresenceofa140K-daltoncomponentcorrelatedwiththepresenceofCDSinembryonalcarcinomacells,whilethepresenceofa150K-daltoncomponentcorrelatedwiththepresenceofCDSinfibroblasticcells.TheseresultssuggestthatCDSinembryonalcarcinomaandfibroblasticcellscomprisedistinctmolecules.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
