Sorting out of coaggregated brain cell types mediated by specific cell recognition factors in vitro: I. Experimental histogenesis and quantitative analysis☆
[摘要] Tworegion-specificcellrecognitionfactors(CRF,cerebrumspecific,andOTF,optictectumspecific),whichmarkedlyincreasedthesizeofaggregatesformedbydissociatedcellsofhomologoustype,havealsobeenshowntopromoteselectiveassociationofhomologouscellswithincoaggregatesformedfromcommingledcerebrumandtectumcellsuspensions.Theresultingpatternsofcellsortingwereexaminedquantitatively.Nearest-neighboranalysisofcellassociationswithinequatorialhistologicalsectionsofthesecoaggregatesevaluatedtheenhancedassociationofregionallyhomologousbraincelltypesinresponsetoeachspecificbraincellrecognitionfactor.Thestatisticalanalysisalsoexaminedacharacteristicpositionalsegregationofcerebrumandtectumcellsinthesortedoutcoaggregates:tectumcellstendedtosegregatetowardtheperipheryofthecoaggregatewhilecerebrumcellsmassedinthecore.Thesedatademonstratethatthecellsofeachpopulationassociatedpreferentiallywithoneanotherindirectresponsetotheirhomologouscellrecognitionfactor.Theapplicationofthisquantitativenearest-neighboranalysisformakingassessmentsofassociativeaffinitiesbetweenneuralcellpopulationsisevaluatedandimplicationsofusingthisanalysisforstudyingregionalandfunctionalhomologybetweennervecellsduringdevelopmentarediscussed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
