Localization of surface and internal acetylcholine receptors in developing fast and slow muscles of the chick embryo☆
[摘要] Thelocalizationofsurfaceandinternalacetylcholine(ACh)receptorswasinvestigatedinthedevelopinganteriorandposteriorlatissimusdorsi(ALDandPLD)musclesinthechickembryo(11,15,and19days)byautoradiographyusing125I-α-bungarotoxin(BTX).At11days,AChreceptorswerealreadypreferentiallyatneuromuscularjunctions.InternalAChreceptors,measuredusingmusclesmadepermeabletoBTXbysaponintreatment,werescatteredthroughoutthelengthofeachmusclefiberwithorwithoutaslightincreaseintheirnumberaroundneuromuscularjunctions.Quantitativeanalysisofgrainsinmontageelectronmicrographsofmusclefibersfrom11-dayembryosrevealedthatintracellularspecificBTXbindingsitesweretheGolgicomplexandmultivesicularbodies.ThenumberofsilvergrainsovertheGolgicomplexdecreasedgreatlyafterpuromycintreatmentoforgan-culturedmuscles.ThesefindingsstronglysuggestthattheGolgicomplexisoneofthesitesinvolvedintheproductionofAChreceptorsintheskeletalmusclecellsinvivo.MultivesicularbodiesareassumedtobeinvolvedinthedegradationofAChreceptors.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
