Quantitative changes in the expression of histone H1 and H2B subtypes and their relationship to the differentiation of mouse embryonal carcinoma cells☆

[摘要] Thepatternofhistonesfromseveralmouseembryonalcarcinomacell(ECC)lines,differentiatedcelllines,andadultorganswasanalyzedusingacid-ureagelscontainingTritonX-100andlongSDS-gelelectrophoresis.AllcelllineshadcomparablehistonetypesexceptforauniqueH2B-likecomponentthatwasfoundonlyintheECClinePCC4.ThemousehistoneH1hasfourdifferentsubtypes(H1a,H1b,H1c,andH1d),asresolvedinSDS-gelelectrophoresis.Theexpressionofthefoursubtypeswasshowntobecelllinespecific.SubtypesH1aandH1darepresentinapproximatelythesamerelativeamountsinallcelllinesinvestigated.SubtypeH1bisfoundinhigherrelativeamountsthansubtypeH1cinECClinesandtestis.TheratioofH1bandH1cisreversedindifferentiatedcelllinesandinkidney,whitebloodcells,liverandspleen.AllfoursubtypesofH1arephosphorylatedalthoughtoadifferentextentindifferentcelllines.InECClines,subtypesH1bandespeciallyH1dincorporatemostofa32Plabel,whereasH1cispredominatelyphosphorylatedindifferentiatedparietalendodermcelllines.ThesedataindicatethatH1subtypesdifferdependingonthestageofcelldifferentiation.DifferenceinratiobetweenH1subtypesandinphosphorylationmightinfluencethechromatinconfigurationandthusgeneexpressioninthesecells.

[学科分类] 生物科学（综合）