An essential role for putrescine biosynthesis during meiotic maturation of amphibian oocytes☆
[摘要] TheobjectiveofthisstudywastoinvestigatetheroleofpolyaminesduringmeioticmaturationofXenopusoocytes.Theresultsindicatearapidandsignificantincreaseintheactivityofornithinedecarboxylase(ODC),therate-limitingenzymeinthepolyaminebiosyntheticpathway,duringthemeioticmaturationinducedbyeitherprogesteroneorhumanchorionicgonadotropin(HCG).Thisincreaseintheenzymeactivitywasfollowedbyanaccumulationofputrescinewithoutanyeffectonthelevelsofspermidineorspermine.TheinhibitionofODCactivityandtheaccumulationofputrescinelevelsbyα-difluoromethylornithine(DFMO),acatalyticirreversibleinhibitorofODC,alsoresultedintheinhibitionofmaturationmediatedbyprogesteroneinXenopusoocytes.DFMOcausedaninhibitionofbothmaturationandovulationinducedbyHCGinovarianfragments.Thisinhibitionwasreadilyreversiblebyexogenoussupplyofputrescinetothemedium.Theseobservationssuggestthatputrescineplaysanimportantroleduringthemeioticmaturationofamphibianoocytes.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
