Fusion of chick embryo skeletal myoblasts: Role of calcium influx preceding membrane union☆
[摘要] Extracellularcalciumisrequiredfornormalprogressionthroughtheinitialrecognition-alignmentphaseofmyogenesis.Wehavefoundthatanetcalciuminfluxintofusion-competentmyoblastsisarequisitestepinmembranefusion,inadditiontothepreviouslydemonstratedrequirementforextracellularcalcium.Thisconclusionisbasedonthefollowingevidence:(1)ameasurableincreaseinnetcalciuminfluxoccursjustpriortofusion;(2)ethyleneglycolbis(β-aminoethylether)N,N′-teraaceticacidcanblockthefusionprocessatastageafteralignmentbutpriortomembraneunion;(3)thecalciumionophoreA23187inducesprecociousfusion;and(4)thecalciumchannelblockerD600inhibitscellfusionbutnotalignment.Basedonultrastructuralanalysesofotherinvestigators,wesuggestamechanismwherebytheincreaseinintracellularcalciumcouldtriggermembraneunionbetweentwoaligned,aposed,fusion-competentmyoblasts.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
