Immunofluorescent localization of collagens, fibronectin, and laminin during terminal differentiation of odontoblasts☆
[摘要] ThelocalizationofcollagenstypesI,II,III,IV,laminin,andfibronectinwasanalyzedinmouseembryonicmolarsbyindirectimmunofluorescence.Usingaffinity-purifiedantibodies,alltheseantigensexceptcollagentypeIIweredetectedintoothgermsandparticularlyattheepithelio-mesenchymaljunction.CollagenstypeI,typeIV,andlamininwerelocalizedatthejunctionbefore,during,andafterodontoblaststerminaldifferentiation.ThestainingpatternscorrespondingtotypeIIIcollagenandfibronectinweremodifiedduringthepolarizationofodontoblasts.CollagentypeIIIpresentattheepithelio-mesenchymaljunctioncouldnolongerbedetectedinthisregionwhenodontoblastswerepolarized.Fibronectin,surroundingpreodontoblasts,wasconfinedtotheepithelio-mesenchymaljunctionwhenodontoblastswerefullypolarized.Previousstudieshadshownthatthepresenceofabasementmembraneandassociatedmaterialwasaprerequisiteforthepolarizationofodontoblasts.Therefore,theredistributionofcollagentypeIIIandfibronectinwasdiscussedintermsoffibronectin-collageninteractionsandtransmembranouscontrolofthecytoskeletonactivityinthedifferentiatingodontoblasts.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
