Synapse formation between dissociated nerve and muscle cells in low density cell cultures
[摘要] Someoftheneuronsdissociatedfromembryonicchickspinalcordandmaintainedinlowdensitycellculturesestablishfunctionalcontactswithmusclefibersthathadformedinvitrofrompreviouslyplatedmyoblasts.Exposureofyoungmuscleculturestod-arabinofuranosylcytosineandsubsequentmaintenanceofcombinedneuron-muscleculturesinlowembryoextractmediaeliminatedmostoftheperipheralandcentralsupportingcells.Thesynapsesarecholinergic,andtransmitterreleasewhich,evenintheyoungestculturesisquantal,isregulatedbyCa2+andMg2+.Themajorfunctionaldifferencesfromadultvertebrateneuromuscularjunctionsare:slowsynapticpotentialtimecourse,lowend-platepotentialquantumcontentandminiatureend-platepotentialfrequency,occurrenceofspontaneousend-platepotentials,andlackoffunctionalacetylcholinesterase.Inaddition,directelectricalcouplingbetweennerveandmusclehasbeenobservedinfourcases.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
