Dedifferentiation of iris epithelial cells☆
[摘要] Electronandphase-contrastmicroscopicobservationsdemonstratedthatdepigmentationofirisepithelialcellsinvivoafterlentectomyisprecededbyalterationsofcellshapeandincreasesinmicrofilamentsandmicrotubulesintheperipheryofthecell.Extensivecellprocessesareformed,withtipsbranchingintofinestrandsthatcontainmelanosomes.Themacrophagesinvadingtheirisepitheliumincorporatepiecesofsuchstrands,whicharecomposedofcellmembrane,cytoplasmicmatrix,andmelanosomes.Individualmelanosomesarealsotakenupbymacrophages.Someofthestrandsofiriscellprocessesseemtodegeneratewithintheintercellularspace.Thus,depigmentationofirisepithelialcellsisaccompaniedbylossofasubstantialpartofthecellsurfaceandcytoplasmicmatrixofirisepithelialcells.Measurementsoftheabsolutevolumeofwholeirisepithelialcells,theircytoplasm,andtheirnucleiwereconductedatvariousstagesofdepigmentation.Thesemeasurementsrevealanextensiveincreaseinthevolumeofthecytoplasmprecedingactivationofthecellsurfaceandasignificantreductionincytoplasmicvolumeduringthephaseofextensivedepigmentation.Inthecaseofthenuclearvolume,theextensiveincreasewhichoccursinparallelwiththatofthecytoplasmicvolumeisnotfollowedbyasignificantchangeduringthedepigmentationphase.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
