Kinetic analysis of amino acid pools and protein synthesis in amphibian oocytes and embryos☆☆☆
[摘要] RatesofproteinsynthesishavebeenmeasuredinRanapipiensoocytesandembryosandinXenopusoocytesfromtheincorporationkineticsoftwodifferentconcentrationsofaminoacid.Thismethoddoesnotrequireanindependentmeasurementoftheaminoacidpools,sincethepoolsizecanbecalculateddirectlyfromincorporationdata.Theeffectsoftheconcentrationanddiffusionofinjectedaminoacidonthecalculatedvaluesforaminoacidpoolsizeandflowratearediscussed.Whentheendogenousaminoacidpoolisappreciablyexpandedbytheinjectedaminoacid,thetotalaminoacidpoolintheoocytesorembryosmaybeconsideredastheprecursorpoolforproteinsynthesis.Underthesecircumstances,compartmentationofaminoacidsdoesnotaffecttheresults,exceptwhenlysineisusedastracer.TheratesofproteinsynthesisinovarianoocytesofRanapipiensandXenopuslaevisare18and50–54ng/hr,respectively.InRanapipiens,therateincreases70%duringmaturationandanother50%beforethetwo-cellstage.Finally,therateapproximatelydoublesbetweenthetwo-cellandblastulastages.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
