Analysis of glycosaminoglycans within the extracellular environments encountered by migrating neural crest cells☆
[摘要] Thevertebratetrunkneuralcrestoriginatesasanundifferentiatedcellpopulationwhichsubsequentlydifferentiatesinresponsetoenvironmentalcuesencounteredduringitsmigration.Glycosaminoglycansaremajorcomponentsoftheembryonicextracellularenvironmentwhichhavebeenimplicatedasimportantinthecontrolofseveralmorphogeneticevents.Toexaminethehypothesisthatglycosaminoglycansmayalsoaffectthedevelopmentoftrunkneuralcrestderivatives,glycosaminoglycanswithinrelevantembryonicregionswerecharacterizedbymicro-spectrophotometricmeasurementsofenzyme-sensitivealcianbluestaining.Theresultsindicatethatthetrunkneuralcrestinitiatesmigrationinanextracellularmatrixrelativelyandabsolutelyrichinhyaluronicacid.Bythecompletionofcrestcellmigration,themajorderivativesofthetrunkneuralcrestaresurroundedbyextracellularenvironmentscontainingdifferentandcharacteristicconcentrationsofhyaluronicacidandchondroitinsulfate.Thepresumptivemelanoblastsandsympathoblasts(whichsubsequentlyundergoasecondarymigration)arebothfoundinregionsofmesenchymewhichcontainhigh,althoughdifferent,levelsofextracellularglycosaminoglycans.Incontrast,thenascentspinalganglioncontainsrelativelylittleextracellularglycosaminoglycan.Theseresultssuggestthatglycosaminoglycansmaybeimportantinthedifferentiationofthetrunkneuralcrest.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
