Myosin in primary cultures of hamster heart cells
[摘要] Primaryculturesofneonatalhamsterheartcellscontainedaheterogeneouspopulationofcelltypes.Musclecellsrepresentedonesuchtype,andcouldbeidentifiedbytheirabilitytocontractspontaneouslyincultureandbytheirmorphologywhenexamineddirectlybyphasecontrastmicroscopyorafterstainingwithphosphotungsticacid-hematoxylin.Overgrowthofmusclecellsbymorerapidlydividingnonmusclecellswaspreventedbytreatmentofthecultureswiththemitoticinhibitor,1-β-d-arabinofuranosylcytosine.Themusclecellsincreasedtheirspecificcontentofmyosinoveraperiodof5daysinculture.Theywerethen,however,relativelyimmaturewhencomparedwiththepredominatingcellsin1-day-oldhamsterhearts.Thestructuralandenzymaticpropertiesofpurifiedmyosinfromthecultureswerenotdifferentfromthoseoftheisoenzymetypeofthisproteinnormallyfoundinneonatalhamsterhearts,andnonmusclecellscontributedlittleofthetotalmyosin.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
