Requirement for RNA and protein synthesis for induced regression of the tadpole tail in organ culture

[摘要] AsimplemethodformaintainingthetailofRanatemporariatadpolesforupto8daysinorganculturehasbeendescribed.Regressionoftheisolatedtailwasinducedbytheadditionoftriiodothyroninetotheculturemedium.Therateofregressionandtheincreaseinactivityofsomehydrolyticenzymeswerecomparabletothoseobservedinthetailsofintacttadpolesundergoingmetamorphosis.CulturedtailsarecapableofsynthesizingRNAandproteinasseenfromtheincorporationofH3-labeleduridineandC14-labeledaminoacids.Hormone-inducedregressionwasaccompaniedbyanacceleratedsynthesisofbothRNAandprotein.ActinomycinD,puromycin,andcycloheximideabolishedregressionandincreaseinhydrolaseactivityintheisolatedtailsinducedbytriiodothyronine.Underconditionsinwhichregressionwasinhibited,actinomycinDandcycloheximideblockedtheincorporationofH3-uridineintoRNA,andpuromycinandcycloheximidethatofC14-aminoacidsintoprotein.AcontinuousgenerationofRNAandproteinisassociatedwiththehormone-inducedregressionoftheisolatedtadpoletail.

[学科分类] 生物科学（综合）

[关键词] T3;3;5;3′-triiodo-l;RNA;ribonucleic acid;DNA;deoxyribonucleic acid;Tris;2-amino-2-hydroxymethyl-1;3-propanediol