Differentiation and competence of cells from hybrid embryos☆
[摘要] CellsfromthelethalhybridgastrulaRanapipiens♀×Ranasylvatica♂wereculturedinvitroassmallaggregates.Thesecellssurviveandundergoextensivedifferentiation,whereasleftwithinthearrestedgastrulatheydie.Incompatibilitybetweennucleusandcytoplasmofthisdiploidhybridappearstoresultinproductionofinhibitorswithintheembryowhichareatleastpartiallylosttothemediumwhensmallcellaggregatesarecultured.Competenceofhybridpresumptiveepidermiscellsforinductionofmelanoblastswasfoundtobelowerquantitativelythancompetenceofnormalcells.Hybridmarginalzonecellsdifferfromnormalschieflyintypeofmusclethatdifferentiates.Functionalcardiacmuscleinhybridssupplantsthevoluntary,striatedmusclecharacteristicofnormals.CellsfromthishybridembryobehavedifferentlyfromnormalRanapipienscellsduringoperationalprocedures,anddifferentclutchesofhybridembryosvaryastoextentofgastrulationandfurtherdevelopmentbeforeonsetofarrest.CellsfromthelethalhybridRanapipiens♀×Ranaclamitans♂wereunabletosurviveinculture.Theblocktodevelopmentatacellularlevelinthiscrossismanifestevenasearlyaslatecleavage.Cellsfrompigmentpatternmutantswereexaminedfortheircompetencetorespondtoaninductorofmelanocytes.BothRanaburnsiandRanakandiyohipresumptiveepidermiscellscanbeinducedtoformextensiveculturesofpigmentcells.Cellsfromthedoubledominant,however,arelessresponsivetotheinductor.Theuseofhybridembryostostudytheintracellularbasisforcompetenceisdiscussed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
