Localization of glucosamine-incorporating materials at epithelial surfaces during salivary epithelio-mesenchymal interaction in vitro☆
[摘要] Submandibularsalivaryepitheliumofthe13-daymouseembryo,followingtrypsin-pancreatinseparationfromitsinvestingmesenchyme,incorporatestritiatedglucosaminewhenincubatedinlabeledtissueculturemediumfor1hourat37°C.Afterbeingculturedfor24hourstrans-filterfromunlabeledmesenchymeofasimilarage,thelabelismanifestedautoradiographicallyashighgraincountbeyondthebasalsurfaceoftheepithelialcells.Graincountsovertheepithelialcellswerefoundtorisetoamaximumbetween2and6hoursincultureandsubsequentlytofall.Graincountsbeyondthebasalcellsurfacewerefoundtoincreaseduringthefirst24hoursinculture.Thesedataareinterpretedtoindicatethatlabelappearingatthebasalcellsurfaceby24hoursinculturerepresentsanepithelialcellproduct.Suggestiveevidencethatthelabeledmaterialisamucopolysaccharidecomesfromthefactthathyaluronidaseremovesthelabelwhereascollagenasedoesnot.Evidenceispresentedthatsurfacematerialsremovedbytrypsin-pancreatinatthetimeofculture,orafter24hours,areimportantincontrollingtheuptakeoftritiatedglucosamine.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
