Lactic dehydrogenases of the developing cerebral cortex and liver of the mouse and guinea pig☆
[摘要] Usingcelluloseionexchangersandstarchgelelectrophoresis,fourcomponentsofLDHhavebeenfoundinthecerebralcortexofmouseandguineapig.TheseveralcomponentstestedcouldalsobedistinguishedbytheirratiosofratesofreductionofDPNandtwoofitsanalogs.ThesecomponentsapparentlyeachcontributetoadifferentdegreetotheincreaseinLDHactivityofthecortexwhichoccursduringdevelopment.MaturationoftheliverisaccompaniedbyareductioninthenumberofcomponentsofLDHinbothmouseandguineapig.Withtheliverofthemouse,peaksofLDHactivityinthechromatogramswhichcorrespondedintheirpositionwiththosefromcerebralcortexalsogavethesameratiosofreductionofDPNanditsanalogs.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] LDH;lactic dehydrogenase;TSM;Tris [tris-(hydroxymethyl)aminomethane];succinic acid;and magnesium acetate in molar concentration ratios of 10:4:5;the stated molarity of the buffer used referring to that of Tris in the mixture;DEAE-cellulose;diethylaminoethyl cellulose;CM-cellulose;carboxymethyl cellulose;APDPN;3-acetylpyridine analog of DPN (diphosphopyridine nucleotide);Py3AlDPN;pyridine-3-aldehyde analog of DPN [时效性]
