Transplantations of blastula nuclei into activated eggs from the body cavity and from the uterus of Rana pipiens: I. Evidence for fusion between the transferred nucleus and the female nucleus of the recipient eggs☆

[摘要] 1.1.Transfersofblastulanucleiweremadeintoactivatedbodycavityanduterinehosteggsinwhichthefemalechromatinwasnotremoved.Informationobtainedontheearlycleavagehistories,polarbodyformation,andploidyoftheresultingembryosindicatethatinthemajorityofcasesafusionhadoccurredbetweenthetransplantednucleusandthefemalepronucleusoftheactivatedegg.2.2.Diploidnucleartransfersintoactivatedbodycavityanduterineeggsgaverisetolarvaethatvariedintheirchromosomenumberrangingfromhaploidtohexaploid.Mosaicswerealsoobtained.2.1.a.Themajorityofembryoswerefoundtobetriploid,andthesedevelopedonlyfromactivatedeggsthatcleavedontime.2.2.b.Hexaploidembryosdevelopedonlyfromactivatedeggswhosefirstcleavagewasdelayedonecleavageinterval.3.3.Haploidnucleartransfersintoactivatedbodycavityeggsgaverisetotadpolespossessingthefollowingchromosomenumbers:haploid,diploid,triploid,andtetraploid;amosaicwasalsoobtained.3.1.a.Themajorityofactivatedeggsthatcleavedontimedevelopedintodiploidembryos.3.2.b.Thetetraploidsandtriploidsdevelopedonlyfromactivatedeggswhosefirstcleavagewasdelayedonecleavageperiod.4.4.Thechromosomenumbersofsomeofthemosaicembryosalsoindicatethatadelayedfusioninvolvingthetransplantednucleusmaytakeplaceinactivatedhosteggs.

[学科分类] 生物科学（综合）