The inhibition of lens-inducing capacity of the optic vesicle with adult lens antisera

[摘要] 1.1.Opticvesiclesremoved,freeofectoderm,fromchickembryosof4–7somiteswereexplantedfor18hoursinamediumcontaininga1:20dilutionoftheglobulinfractionofanti-adultlenssera.Thevesicleswerethenenclosedincompetentectodermandcultured3–4daysinamediumfreeoftheantisera.Inductionofalensoccurredin2of61cases(3%).2.2.Inductionofalensresultedin35of66cases(53%)inthecontrolvesiclesculturedina1:20dilutionofnormalrabbitsera(globulinfraction)andsubsequentlyenclosedinectoderm.3.3.Theresultsofexperimentsinwhichopticvesicleswereculturedinmediacontainingeitherantilensseraornormalrabbitseraandsubsequentlyimplantedbeneaththeectodermof4–7somiteembryosconformtotheresultsoftheinvitroexperiments.4.4.A1:20dilutionoftheanti-adultlensserafailedtoinhibitdevelopmentoftheoticvesicle.5.5.Sectionsofselectedstagesofnormalembryoswerestainedwithantilensseraconjugatedwithfluoresceinisocyanate.Resultsindicateareactionofsubstanceswithintheopticvesiclewiththefluoresceinconjugatedlensantiserapriortolensinduction.6.6.Theinhibitionofthelens-inducingcapacityoftheopticvesiclebytheanti-adultlensserasuggeststhatsubstanceswithintheopticvesicle,whichcanreactwithadultlensantibodies,areessentialintheinductiveprocess.

[学科分类] 生物科学（综合）