Drosophilia myogenesis in vitro: The genesis of “small” myocytes and myotubes☆

[摘要] CellcultureswerepreparedfromDrosophilamelanogasterembryos35minutesaftertheinitiationofgastrulation.Individualcellswereobservedatintervalsbyphasecontrastmicroscopy,andadescriptionofmyogenesiswasassembled.Typically,amesenchyme-likemyoblastdividedinvitroabout5hraftertheinitiationofgastrulation.Thedaughtercells(“small”myocytes)aggregatedwitheachotherandwithneighboringmyocytesduringthenext7hr,approximately,butdidnotdivideagain.Atabout12hraftertheinitiationofgastrulation,themyocytesbegantoelongate.Duringthenextseveralhourstheycompletedelongation,becamebipolarandsometimespulsatile.Aggregatedmyocytesalignedthemselvesinlineararrays,andsomeofthemfusedtoformmyotubes.Thedifferentiationof“small”myocytesandmyotubesderivedfromthemwasapparentlyautonomous,beginningwiththemyoblaststageimmediatelyprecedingdivisionatabout5hr,anditmightbeautonomousfromevenearlierstagesofmyogenesis.

[学科分类] 生物科学（综合）