The avoidance of stimulatory artifacts in cell cycle determinations in vitro☆
[摘要] MyogenicculturesexhibitapredictablepatternofgrowthcharacterizedbyaninitiallyexponentialaccumulationofDNA,whichdeclinestoaplateaulevelafterfusion.Thispatternisrelatedtocell-mediatedmodificationsoftheculturemediumandcanbealteredbydeviationsfromtheroutinefeedingschedule.ThedatademonstratethatfreshmediumstimulatesDNAaccumulationinoldercultureswhilethemediumremovedfromsucholder,fusingculturesreducestherateofDNAaccumulationinyoungerculturesandalsopromotesfusion.Underexperimentalconditionswhichrequiresomereplacementofmedium(e.g.,pulse-chaselabeling),sucheffectsarepotentialsourcesofexperimentalartifact.Suchartifactscanbeeliminated,however,bytheproperchoiceofmedium.Theleastperturbationoftheculturesoccurswhenmediumwithdrawnfromsisterculturesofthesameageisemployed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
