Freeze-cleave demonstration of gap junctions between skeletal myogenic cells in vivo☆
[摘要] Developingmusclemassesfromhindlimbsof19-dayfetalratswerefreeze-cleaved,platinumandcarbonreplicated,andexaminedelectronmicroscopically.Gapjunctionswereobservedlinkingcellpairsclearlyidentifiedasmyogenicbythepresenceofeasilyrecognizedandcharacteristicarraysofcrossorlongitudinallyfracturedmyofibrils.Occasionallygapjunctionswerealsoobservedbetweenidentifiednonmyogeniccells,butnonewereobservedbetweenmyogenic-non-myogeniccellpairs.Becausetherecentlyformedconjointmyogeniccellswerealreadyencapsulatedbydevelopingbasallaminaeandnormallywouldhavefusedtoformdiscretemyofibers,wesuggestthatthisreportprovidesadditionalevidencethatgapjunctionsnormallyformimmediatelybeforeandthusperhapsmediatetheinitialeventsofmyogeniccellfusioninvivoaswellasinvitro.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
