Preferential synthesis of actin during early development of the slime mold Dictyostelium discoideum☆
[摘要] ThechangesinproteinsynthesisduringdifferentiationofthecellularslimemoldDictyosteliumwerestudiedbySDS-polyacrylamidegelelectrophoresis.Totalcellproteinwasanalyzedfollowinga2-hrpulse-label.Itwasfoundthatduringthepreaggregationstage,comprisingthefirstthirdofthedevelopmentalcycle,asinglemajorbandaccountsformorethan20%ofthetotallabeledproteinonthegel.Thisspecieswasproducedinatleast5–10-foldloweramounts,relativetototalcellproteinsynthesis,invegetativecellsandinlaterdevelopingstages.Actinwaspurifiedfromvegetativecellsandwasfoundtocorrespondtothemajorbandinseveralrespects.ThediscoveryofasingleproteinbeingsynthesizedinsuchquantityataspecificdevelopmentalstageprovidesapowerfultoolfortheisolationofaspecificmessengerRNAmoleculeandforanintensivestudyofallthefactorsinvolvedinregulatingproteinsynthesisinaeukaryoticorganism.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
