Depression by hyaluronic acid of glycosaminoglycan synthesis by cultured chick embryo chondrocytes☆
[摘要] Theeffectsofhyaluronicacidontheexpressionofdifferentiationbypurepopulationsofculturedchickembryochondrocytesarestudied.Inmediumcontainingserum,hyaluronicacidhaslittleeffectonsulfateincorporation.Intheabsenceofserum,200μg/mlofhyaluronicacidinhibitssulfateincorporationintohyaluronidase-sensitivematerialassociatedwiththecelllayerbyasmuchas50%.Thiseffectisseenby6hrandisprogressive.Hyaluronicacidhasnoeffectonleucineorthymidineincorporationorsulfateuptake,matrixturnover,orcollagensynthesisunderthesesameconditions.Itisshownthathyaluronicacidinhibitschondroitinsulfatesynthesisandtheaccumulationofnewlysynthesizedproteoglycaninthecelllayer.Theseeffectsarenotproducedbyotherglycosaminoglycans,suchaschondroitinsulfate,keratansulfate,orheparansulfate,orbymonosaccharides,butoligosaccharidesderivedfromhyaluronicacidmimictheeffectofhyaluronicacid.Thesedataindicatethatspecificglycosaminoglycans,suchashyaluronicacid,canaffectthesynthesisandaccumulationofotherglycosaminoglycansandshouldbeconsideredaspossibleregulatorsofspecificcellularactivities.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
