The role of acid phosphatase and β-glucuronidase in reopening and remodeling of the developing chick esophagus☆☆☆
[摘要] Biochemicalandultrastructuralmethodshavebeenemployedtodeterminetherelationshipofthelysosomalsystemtoreopeningandremodelingofthedevelopingchickesophagus.Biochemicaldataindicateanincreaseinbothacidphosphataseandβ-glucuronidaseactivityduringesophagealorganogenesis.Theappearanceofthesehydrolaseenzymeactivitiesappearstobeindependentlyregulated.Theincreaseinacidhydrolaseactivitiesparallelstheincreaseintotalproteinatatimewhentheesophagusisgreatlyenlarging.Thegreatestactivityofacidphosphatasewaslocalizedintheanterioroccludedsegmentoftheesophagus.Withreformationofthelumen,thedistributionofacidphosphatasebecomesuniformalongtheorgan.Increasesinhydrolaseactivitiescorrelatecloselywithcelldegenerationandthedifferentiationoftheepithelium.AcidphosphataseisidentifiedcytochemicallyintheGolgicomplexandlysosomesofembryonicepithelialcells.Surroundingmesenchymecontainsonlymodestcytochemicalactivity.DiffuseGolgicomplexesandprimarylysosomespredominateduringearlystagesstudied.SecondarylysosomesandcondensedGolgicomplexesarecharacteristicofdifferentiatingepitheliumatlaterstages.Acidphosphataseactivityappearsselectivelylocalizedinvitalepithelialcells,whiledegeneratingandmoribundcells,whoseappearancesareobligatorytothereopeningprocess,failtodemonstrateenzymaticactivity.Thetemporalassociationofincreasedhydrolaseactivityandcytochemicalmaturationofalyosomalsystemsuggestsanactiveinvolvementofthesysteminesophagealorganogenesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
