Genetic, cytological, and ultrastructural characterization of a temperature-sensitive lethal in Drosophila melanogaster☆
[摘要] GeneticanalysisofastrainofDrosophilamelanogasterrevealedthatarecessivemutation[l(1)ERts]causingtemperature-sensitiveembryoniclethalityislocatedinthedistalregionoftheXchromosomeapproximatelyatmapposition18.At22–25°Cmutantembryosexhibitnormalviability,andalleggsarrestpriortogastrulationiftheyarerearedat29°C.Themutantisbiphasic,exhibitingamaternaleffectwhichisexpressedthroughoutthefirst8hrofdevelopmentaswellasasecondtemperature-sensitiveperiod(TSP)duringthefirst3daysoflarvallife.Larvaeexposedtotherestrictivetemperature(RT)duringthesecondTSPmustalsospendtheremainderoflarvalandpupallifeandthetimeofnormaleclosionatRTtodieasfullydevelopedpupaewhichfailtoeclose.Lightandelectronmicroscopyofarrestedembryosrevealdisturbancesinthedistributionofnuclei,cytoplasm,andyolkandabnormalconfigurationsofroughendoplasmicreticulum.Thecauseofpupaldeathduringthesecondlethalperiodisunknown.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
