Mechanism of soybean trypsin inhibitor induced polyspermy as determined by an analysis of refertilized sea urchin (Arbacia punctulata) eggs☆
[摘要] Soybeantrypsininhibitor(SBTI)retardstheseparationofthevitellinelayerfromthesurfaceofArbaciaeggsandtheformationofthefertilizationmembrane.Numerouslociofvitellinelayer(corticalprojections)remainassociatedwiththeplasmamembraneofSBTI-treatedzygotesimmediatelyafterinsemination.Graduallytheattachedportionsofvitellinelayerdecreaseinnumberandbyapproximately15minpostinseminationthecorticalprojectionsaresparseornonexistent.MonospermicSBTI-treatedzygotesshowacorrespondingdeclineinboththeincidenceanddegreeofrefertilization(polyspermy)uponreinseminationduringthisperiod.UltrastructuralanalysisdemonstratesthatrefertilizingspermatozoaareabletofusewithandpenetrateSBTI-treatedzygotesonlyattheapicalsurfaceofthecorticalprojections,i.e.,wherethevitellinelayerremainsattachedtotheplasmamembraneofthezygote.Thesedataindicatethatthevitellinelayerliningtheapicalsurfaceofthecorticalprojectionsretainsstructuralandfunctionalpropertiescharacteristicofthesurfaceoftheunfertilizedovum.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
