The effect of β-ecdysone on protein synthesis in imaginal discs of Drosophila melanogaster cultured in vitro: I. The effect on total protein synthesis☆
[摘要] β-Ecdysoneincreasestherateofincorporationof3H-leucineintoproteininimaginaldiscsofDrosophilamelanogasterculturedinvitro.Ananalysisoftherateofturnoverofnewlysynthesizedproteinandthespecificactivityoffree3H-leucineindiscsexposedandnotexposedtoβ-ecdysoneindicatesthattheincreasedincorporationof3H-leucineresultsfromanincreaseinproteinsynthesis.Theconcentrationoffreeintracellularleucineindiscsisfoundtobepredominantlyregulatedbytherateoftransportofleucineintodiscsandtherateofremovalbyproteinsynthesis.Theincreasedlevelofproteinsynthesisinducedbyβ-ecdysoneismaintainedfollowingremovalofthehormonefromtheculturemedium.Theavailableevidenceindicatesthatβ-ecdysoneactsonthegenomeofdisccellstobringabouttheincreasedrateofproteinsynthesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
