Clonal analysis of vertebrate myogenesis: II. Environmental influences upon human muscle differentiation☆
[摘要] Invitroproceduresforobtainingthedifferentiationofhumanfetalmusclecoloniesweredeveloped,andthesensitivityofclonaldifferentiationtoenvironmentalinfluenceswasexamined.Humanmusclecoloniesarecapableofdifferentiatingintheabsenceofanexogenouscollagensubstrate.Thedependenceofclonaldiffeentiationupontheadditionofchickembryoextracttotheculturemediumisdeterminedbytheserumtypeusedinthemediumandbythesubstrateuponwhichthecoloniesaregrown.Clonaldifferentiationalsodependsuponconditioningofthemediumbythecolonies.Therateofmediumconditioningisaffectedbyclonaldensityandinitialmediumcomposition.Therequiredmediummodificationisnotspeciesspecificsincemediumconditionedbychickmusclecellsalsopermitstheearlydifferentiationofhumanmuscleclones.Bymanipulatingthevariousenvironmentalparametersdescribedaboveithasbeenpossibletodefineanumberofinvitroconditionswhichpermitanormalrateofcellproliferationbutdonotpermitcellfusion.Resultsfromtheseexperimentsarediscussedintermsoftheirdevelopmentalimplications.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
