Restoration of fertility in sterilized Drosophila eggs by transplantation of polar cytoplasm☆
[摘要] AtechniquefortransplantationofcytoplasmbetweenDrosophilaeggsisdescribed.PolarcytoplasmofnewlylaideggswasfirstmadeineffectiveforthedeterminationofgermcellsbyUVirradiation.ThesterilitywhichresultsfromthisUVirradiationcouldbepreventedbytheinjectionofpolarcytoplasm,butnotbytheinjectionofanteriorcytoplasmfromunirradiateddonoreggs.Theresultsprovidethefirstdemonstrationoftransplantationofagentscausingdeterminationinaninsectandalsoprovideabioassayfortheseagents.MicroscopicalobservationsofUVirradiatedeggswithandwithouttransplantedpolarcytoplasmrevealedthatUVirradiationdelaysthemigrationofcleavagenucleiintotheposteriorperiplasmandpreventscytoplasmicprotrusionsattheposteriorpolefrombecomingisolatedfromtheperiplasmtoformpolecells.Transplantationofpolarcytoplasmrepairsthesedefects.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
