Transmission of radioisotopically labeled materials during embryonic induction in vitro☆
[摘要] Transfiltermorphogenesisofembryonicmousekidneymesenchymeundertheinfluenceofspinalcordlabeledwithtritiatedleucinehasasacorrelatedfeaturethefixationofradioactivematerialinafiltermembraneseparatingthetissues.Inductionofkidneytubulesinthemesenchymedoesnotoccurwhenthetissuesareseparatedbytwofilterlayerswithaninterveninglayerofcellophane.Autoradiogramsshownosignificantgraincountsinthemesenchyme-associatedfilteralthoughthemesenchymeisweaklylabeled.Morphogenesisofthemesenchymedoesoccurifthecellophanelayerhasasmallhole.Autoradiogramsofsuchculturesshowsignificantgraincountsinthemesenchyme-associatedfilterjustabovetheholeandhighergraincountsoverthemesenchymeabovetheholethanovermoreperipheralmesenchyme.Additionalcorrelationsbetweenthebehaviorofthefilter-fixableradioactivematerialandtheinductiveactivityhavebeenderivedfromstudiesofdifferentmassesoflabeledspinalcord.Reductioninthemassoftheinducingtissueleadstocorrespondingreductionsinthenumberoftubuleswhichcanbeinduced,reductioninthemaximumdistanceoverwhichthelabeledspinalcordcanexertitsinductivestimulus,andreductioninthedistanceoverwhichsignificantgraincountscanberecordedawayfromagivenmassoflabeledinducingtissue.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
