Sperm-induced protrusions in sea urchin oocytes: A study of phase separation and mixing in living cytoplasm
[摘要] ThephenomenadescribedbyAlbertsson(1960)asoccurringinphasesystemsareregardedasusefulmodelsfortheunderstandingofbiologicalstructure.Asurveyisgivenofresultsinrespecttophaseseparationandpartitionwhichareobservedinfertilizedseaurchineggsafterpretreatmentwithlowdosesoftrypsin.Uponinseminationofseaurchinoocytes,thespermatozoainducetheformationofprotrusions(receptioncones).TheseconsistofgroundcytoplasmandformaphaseIstrictlyseparatedfromamoreproximalphaseII,richindispersedendoplasmicvesicles.TherestofthecytoplasmisrichingranularcomponentsandmayberegardedasaphaseIII.Thisviewissubstantiatedbyobservationsontheremixingofcomponentsthatoccursduringthewithdrawaloftheprotrusion.Thisisaprocesswhichisdrivenbythetendencytoremixandbyactivemovementsoccurringintheprotrusions.Thesuggestionismadethatthegroundcytoplasmconsistsofseveralphasesthatundernormalconditionsaremiscibletovaryingextentsaccordingtotheconditionsprevailing.Theformationofprotrusionswithensuingreversiblephaseseparationisregardedasduetoabasicprotectivemechanismoperatinginthecytoplasm.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
