The effect of glucose on respiration of the differentiating slime mold
[摘要] TherespirationofthedifferentiatingslimemoldDictyosteliumdiscoideumatdifferentstagesinitslifecyclewasmeasuredwithandwithoutanexogenousenergysource.Itwasfoundthatonadryweightbasis(microlitersoxygenperminutepermilligramdryweight)respirationishighestattheamebastage,declinestoaplateaufromthemigratingpseudoplasmodiumthroughtheculminationstages,anddropsagainatthesorocarpstage.Glucosestimulatesrespirationmaximallyatthesorocarpstage,inspiteofthefactthatthecellsaremaximallypermeabletoglucoseatpreculmination.TheQO2valuesobtainedforsorocarpsinthepresenceofglucoseequaledthoseobtainedforthepseudoplasmodiumandpreculminationstagesintheabsenceofglucose.TheabsoluteoxygenuptakeofagroupofcellsdifferentiatingonagarinaWarburgflaskwasmaximalattheamebastageanddroppedsharplyduringpseudoplasmodiumformation.Alineardecreaseintotaloxygenconsumedwasobservedfromthemigratingpseudoplasmodiumtothefruitstage.Consideredtogether,thedatasuggestthatnosignificantincreaseinrespirationoccursduringtheculminationprocessandthatacriticaldepletionofendogenousenergysourcesoccursduringdevelopmentatculmination.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
