A Transgenic Lef1/β-Catenin-Dependent Reporter Is Expressed in Spatially Restricted Domains throughout Zebrafish Development
[摘要] TheWnt/β-cateninsignalingpathwayplaysmultiplerolesduringembryonicdevelopment,onlyafewofwhichhavebeenextensivelycharacterized.AlthoughdomainsofWntexpressionhavebeenidentifiedthroughoutembryogenesis,anatomicalandmolecularcharacterizationofrespondingcellshasbeenmostlyunexplored.Wehavegeneratedatransgeniczebrafishlinethatexpressesadestabilizedgreenfluorescentprotein(GFP)variantunderthecontrolofaβ-cateninresponsivepromoter.Earlyzygoticexpressionofthistransgene(TOPdGFP)mirrorsknowndomainsofWntsignalingintheembryo.LossofLef1activityresultsindecreasedreporterexpressionandposteriordefects,whilelossofTcf3(Headless,Hdl)activitydoesnotalterreporterexpression,eventhoughitresultsinlossofforebrainstructures.Inaddition,ectopicWnt1expressioncanactivatethereporter.Inolderembryos,weidentifyanumberoftransgene-expressingcellpopulationsasnovelsitesofβ-cateninsignaling.WeconcludethatourTOP-dGFPreporterlinefaithfullyillustratesdomainsofβ-cateninactivityandenablestheidentificationofresponsivecellpopulations.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] Wnt;β-catenin;Lef1;Tcf3;GFP;reporter;transgenic;zebrafish [时效性]
