Some factors associated with a developmental change in hemoglobin components in early fetal mice☆☆☆
[摘要] HemoglobinsolutionsobtainedfromfetalC57BL6Jmiceseparateintofourbandsuponelectrophoresisinstarchgel.Usingthinstripsofstarchgelandadensitometer,wemeasuredtherelativeconcentrationsofeachofthesebandsinsolutionsderivedfromfetalredcellscollectedfromanimalsof12to17daysofgestation.Wealsomeasuredtheradioactivityofbandsinstarchgelsrunwithsolutionsobtainedfromfetalbloodcellsof12to15daysofgestationfollowingincubationina59Fe-containingculturemediumfor6-hourperiods.Ourresultsshowthatband1,whichhastheelectrophoreticcharacteristicofadulthemoglobin,increasessharplyinrelativeconcentrationandactivitythroughouttheperiodstudied.Bands2,3,and4arecharacteristicoffetalblood.Thesebandsdecreaseinbothconcentrationandactivityatdifferingrates.Band4inparticularhasarateofironincorporationwhichisalwaysmuchhigherthanmightbeexpectedfromestimatesofitsrelativeconcentration.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
