Hemopoietic spleen colony studies: I. Growth and differentiation☆
[摘要] Thegrowthanddifferentiationofspleencolonies(discretenodulesofhemopoietictissuearisinginthespleensoflethallyirradiatedmicegivenlowdosesofisogenicbonemarrowcellsintravenously)arediscussed.Criteriaforcountingandidentifyingcolonies,adescriptionofeachofthedifferenttypesofcolonies,andtheirrelativeincidence,sizeandlocationareconsidered.Erythroid,neutrophilic,megakaryocyticandeosinophilichemopoieticcellshaveeachbeenfoundintheformofgrossormicroscopicspleencoloniesofsingledifferentiatedcelltype.Eachofthespleencoloniesinitiallyundergoesdifferentiationintoasinglelineofhemopoiesis.Inthespleen,erythroidcoloniesarebyfarthemostnumerous,followedbyneutrophilicandthenbymegakaryocyticcolonies,undifferentiatedcoloniesbeinguncommonandeosinophiliccoloniesrare.Theincidenceofmixedcoloniesincreaseswithtimeascolonieswithasinglelineofdifferentiatedcellsenlargeandundergosecondandthirdlinesofhemopoieticdifferentiation.Colonysizeandcelltypeshowasignificantcorrelation.Mixedcoloniesarethelargestinsize.Nextlargestareerythroidcolonies,thenneutrophiliccolonies.Usuallytoosmalltobegrosslyvisiblearetheundifferentiatedcolonies,eosinophiliccoloniesandmegakaryocyticcolonies,indecreasingorderofsize.Evidenceiscitedfromthisandotherworksuggestingthatthetypicalcolonyformingunitisasinglepluripotentstemcell,whosecommitmenttoalineofdifferentiationisinfluencedbymicroenvironmentalinfluencesaswellassystemichumoralfactors.
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[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
