Autoradiographic study of the incorporation of uridine-3H during myogenesis in tissue culture☆
[摘要] Autoradiographicinvestigationofdifferentiatingthighmuscletissueculturesshowedthatafterbriefexposurestoradioactiveuridineorcytosine,theamountoflabelingofnucleiwithinmusclefiberswassignificantlylowerthanthelabelingofnucleiwithinthemononucleatedcells.Thedecreaseintherateoflabelingwhichoccurredattheonsetoffusionofthemyoblastintomultinucleatedfiberscannotbeascribedtogreaterabsorptionoftheβraysbythecytoplasmofdifferentiatedcellsnorisitduetolackofpenetrationofthelabeledprecursorintothecells.ThedifferenceintheincorporationofRNAprecursorsalsodoesnotseemtoarisefromdifferencesinthemitoticactivityofthevariouscelltypes,sinceinhibitionofmitosisbyX-irradiationdidnotabolishthedifferentialrateofincorporationofuridineintoRNA.Theincorporationof3H-leucine,unlikethatofradioactiveuridine,doesnotseemtodecreasefollowingcellfusion.ApplicationofactinomycinDresultedinahigherinhibitionofaminoacidincorporationintomononucleatedcellscomparedwiththatintomultinucleatedfibers.ThesignificanceoftheseresultsinrelationtothepossibleformationofastabletypeofRNAduringdifferentiationisdiscussed.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
