Protein synthesis in sea urchin eggs: I. Fertilization-induced changes in subcellular fractions☆
[摘要] Ribosomalpelletandsupernatantfractionsseparatedbyhighspeedcentrifugationfromunfertilizedandfertilizedseaurchineggshavebeenrecombinedwitheachotherandwithtotalhomogenateandtestedfortheircapacitytoincorporatevaline-14Cintoprotein.Theribosomalpelletfromthefertilizedeggwasalwaysactivewhentestedwitheitherunfertilizedorfertilizedeggsupernatantorwithunfertilizedeggtotalhomogenate.Ontheotherhand,theunfertilizedeggribosomalfractionwasalwaysinactive.Theunfertilizedeggsupernatantwaslessefficientthanthesupernatantderivedfromfertilizedeggswhentestedwithfertilizedeggribosomalpellets.Thisrelativelowerefficiencycouldbeattributedinparttotheloweractivityofaminoacyl-RNAsynthetaseandtotheunavailabilityofsRNAintheunfertilizedegg.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] [时效性]
