Regeneration and division in Stentor coeruleus: the effects of microinjected and externally applied actinomycin D and puromycin☆

[摘要] ConditionsforinhibitionoforalregenerationinStentorcoeruleusbyactinomycinDandpuromycinhavebeenestablished.Asolutionofactinomycin,50μg/ml,appliedexternallyfor48hoursblockedregeneration,theeffectbeingreversibleinpondwaterafter72hours.Wheninjectedpriortostage4,inhibitionofactinomycinwasimmediateandirreversible,butregenerationwasindependentoftheactionofthedrugafterstage4,althoughsubsequentregenerationsequenceswereblocked.Puromycinat150μg/mlblocksregenerationwhenappliedexternallybeforestage6,thedrugreachinginhibitoryconcentrationswithin2hours.Similarly,reversalofinhibitiontook2hours.Puromycininjectedbetweenstages1and5retardedorreversedregeneration,butlaterstageswereinsensitive.Thisinhibitionisalsoreversible.Actinomycin(50μg/ml),puromycin(150μg/ml),andchloramphenicol(500μg/ml)blockeddivisioninstentors.Theeffectsofactinomycinandpuromycinwereirreversible,butanimalstreatedwithchloramphenicolandthenreturnedtopondwaterdividedatahigherratethanthecontrols.Inviewoftheestablishedmechanismsofactionoftheantimetabolites,theseresultsindicatethatremovaloforalstructuresinitiatesactinomycin-sensitiveDNA-templatedRNAsynthesisrequiredduringtheearlystagesofregenerationforprimordiumdevelopment.Newprotein,thesynthesisofwhichisinhibitedbypuromycin,isrequiredduringintermediatestagesofregeneration,butnotduringthelatterpartoftheprocess.

[学科分类] 生物科学（综合）