Regulation of Synaptotagmin Gene Expression during Ascidian Embryogenesis
[摘要] Theascidianembryo,amodelfortheprimitivemodeofchordatedevelopment,rapidlyformsadorsalnervoussystemwhichconsistsofasmallnumberofneurons.Here,wehavecharacterizedthetranscriptionalregulationofanascidiansynaptotagmin(syt)genetoexplorethemolecularmechanismsunderlyingdevelopmentofsynaptictransmission.Insituhybridizationshowedthatsytisexpressedinallneuronsdescribedinpreviousstudiesandtransientlyintheembryonicepidermis.Neuronalexpressionofsytrequiresinductionfromthevegetalsideoftheembryo,whereasepidermalexpressionoccursautonomouslyinisolatedectodermalblastomeres.Introductionofgreenfluorescentproteinreportergeneconstructsintotheascidianembryosindicatesthatagenomicfragmentofthe3.4-kb5′upstreamregioncontainspromoterelementsofsytgene.Deletionanalysisofthepromotersuggeststhatsytexpressioninneuronsandintheembryonicepidermisdependsondistinctcis-regulatoryregions.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] neuronal gene expression;synaptotagmin promoter;ascidian;neural induction;motor neuron;GFP;epidermis [时效性]
