Drosophila APC2 and APC1 Play Overlapping Roles in Wingless Signaling in the Embryo and Imaginal Discs
[摘要] Theregulationofsignaltransductionplaysakeyroleincellfatechoices,anditsdisregulationcontributestooncogenesis.ThisdualityisexemplifiedbythetumorsuppressorAPC.Originallyidentifiedforitsroleincolontumors,APCfamilymembersweresubsequentlyshowntonegativelyregulateWntsignalinginbothdevelopmentanddisease.TheanalysisofthenormalrolesofAPCproteinsiscomplicatedbythepresenceoftwoAPCfamilymembersinfliesandmice.Previousworkdemonstratedthat,insometissues,singlemutationsineachgenehavenoeffect,raisingthequestionofwhetherthereisfunctionaloverlapbetweenthetwoAPCsorwhetherAPC-independentmechanismsofWntregulationexist.WeaddressedthisbyeliminatingthefunctionofbothDrosophilaAPCgenessimultaneously.WefindthatAPC1andAPC2playoverlappingrolesinregulatingWinglesssignalingintheembryonicepidermisandtheimaginaldiscs.Surprisingly,APC1functioninembryosoccursatlevelsofexpressionnearlytoolowtodetect.Further,theoverlappingfunctionsexistdespitestrikingdifferencesintheintracellularlocalizationofthetwoAPCfamilymembers.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] APC;β-catenin;Armadillo;Wnt;Wingless;Drosophila;tumor suppressor [时效性]
