Crossing the Ventral Midline Causes Neurons to Change Their Response to Floor Plate and Alar Plate Attractive Cues during Transmedian Migration

[摘要] Neuronalmigrationisrequiredfortheestablishmentofspecificneuralstructures,suchaslayersandnuclei.Neuronsmigratealongspecificmigratoryroutestowardtheirfinaldestinations,sometimesacrosslongdistances.However,thecellularandmolecularinteractionsthatcontrolneuronalmigrationarelargelyunknown.Here,weexaminedthemechanismunderlyingthetransmedianmigrationofprecerebellarneuronsusingaflatwhole-mountpreparationoftheratembryo.Theseneuronswereinitiallyattractedbythefloorplate(FP)attheventralmidline.However,aftercrossingthemidline,theylosttheirresponsivenesstotheFPandbecameattractedbythealarplate(AP).AlthoughthelossofresponsivenesstoFPcueswascausedbyanencounterofmigratingcellswiththeFP,thegainofresponsivenesstoAPcuesoccurredirrespectiveoftheirencounterwiththeFP.Theseresultsidentifyacrucialchangeintheresponseofmigratingcellstoattractiveguidancecuesduringthetransmedianmigrationofprecerebellarneurons.

[学科分类] 生物科学（综合）

[关键词] tangential migration;transmedian migration;precerebellar neurons;floor plate;alar plate;netrin-1;attraction