N-Twist, an Evolutionarily Conserved bHLH Protein Expressed in the Developing CNS, Functions as a Transcriptional Inhibitor
[摘要] Membersofthebasichelix–loop–helix(bHLH)transcriptionfactorfamilyplayanessentialroleinmultipledevelopmentalprocesses.Duringneurogenesis,positiveandnegativeregulationbybHLHproteinsisessentialforproperdevelopment.HerewereporttheidentificationandinitialcharacterizationofthebHLHgene,Neuronaltwist(N-twist),namedforitsneuralexpressionpatternandhighsequencehomologyandphysicallinkagetothemesodermalinhibitor,M-twist.N-twistisexpressedinthedevelopingmousecentralnervoussysteminthemidbrain,hindbrain,andneuraltube.Thisneuralexpressionisconservedininvertebrates,asexpressionoftheDrosophilaorthologofN-twistisalsorestrictedtothecentralnervoussystem.LikeotherbHLHfamilymembers,N-TwistheterodimerizeswithEproteinandbindsDNAataconsensusbHLH-bindingsite,theEbox.WeshowthatN-TwistinhibitsMASH1-dependenttranscriptionalactivationbysequesteringEproteininadominantnegativefashion.Thus,thesestudiessupportthenotionthatN-Twistrepresentsanovelnegativeregulatorofneurogenesis.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物科学（综合）
[关键词] N-twist;basic-helix–loop–helix;bHLH;achaete-scute;neurogenesis;MASH1;inhibitor;transcription;brain;neural tube;mouse;Drosophila [时效性]
