Colorimetric MTT Assessment of Antifungal Activity of ZnO Nanowires Against Candida Dubliensis Bioflm

[摘要] BackgroundandObjective:Inrecentyearstheincidenceopportunisticfungualinfectionshasincreaseddramatically.OneofthemostcommonfungalpathogenCandidadubliniensis,colonizationandbiofilmformationonthesurfacesofmedicaldevices.TheaimofthisstudywastoevaluatetheinhibitoryactivityofzincoxidenanoparticlesagainstC.dubliniensisbiofilmformationbytheMTTcolorimetricmethodismeasured.MaterialsandMethods:Inthisstudy,ZnOnanopariclesusingthesol-gelwasprepared,sizeandtypeofparticles,respectively,byscanningelectronmicroscopy(SEM)andX-Ray-Diffractionweredetermined.TheMinimuminhibitoryconcentration(MIC)ofgrowthdilutionmethodwasdonebymicrodilutiontest.C.dubliensisbiofilms(DSY1024)after48hincubationat37ºCinhibitedtheinformationofnanoparticlesZnO,SDSandFluconazoledrugasapositivecontrolonCandidabiofilmswasassessedbyMTTtetrasoliumsaltreduction.Thecollecteddatausingstatisticalt-testandSPSSsoftwarewereanalyzed.Results:Theresultsofthisstudyshowedthataminimumconcentrationofinhibitornanoparticles,ZnO,SDSandfluconazole,respectively:9/25,0/02,8µg/ml.InhibitorystrengthofbiofilmadhesioninthepresenceofnanoparticlesZnO,SDSandfluconazole,equivalenttomorethantwicetheconcentrationoftheMICwasdetermined.Conclusion:Inthisstudy,ZnOnanoparticlessynthesizedbyachemicalmethodindicatthathasanti-fungalproperties.Therefore,anewwayofworkingforthepreventionofbiofilmformationofCandidabiofilmsparticularlyassociatedwithmedicaldevicestobe.

[学科分类] 基础医学