The Expression Levels of RAD51 after Ionizing Radiation in Peripheral Blood Lymphocytes for Evaluation of DNA Repair in Breast Cancer Patients Referred to Ahvaz Golestan Hospital

[摘要] BackgroundandObjective:Breastcancerisoneofthemostcommoncancersamongthewomen.Oneoftheradicalmethodfortreatmentofbreastcancerisradiotherapy.Radiationinducesdifferentresponsesincells,includingcellcyclecheckpointactivation,DNArepairandapoptosis.Tocheckcellularresponsesandradiosensitivityineachpatient,DNArepairmechanismandproteins,asbiologicalmarkers,canbeevaluated.BasedontherepaircapacityandradiosensitivityofcellsineachpatientwecandesignatreatmentplanningusingradiationtherapyThepurposeofthisstudywastoevaluatetotheradiosensitivityinbreastcancerpatientstoimprovetreatmentplanningSubjectsandMethods:AquantitativereversetranscriptasePCR-basedassayformeasurementoftheexpressionlevelofRAD51inlymphocytecellsforallbreastcancerpatientsandhealthycontrols.StatisticalanalyseswereperformedusingSPSS17software.TherelationbetweentheexpressionlevelofRAD51andclinicalcharacteristicsofpatientswasanalyzedbyusingthenonparametricMann-WhitneyandKruskal-Wallistests.Results:TheexpressionlevelofRAD51significantlyupregulatedintheperipheralbloodlymphocytesofpatients(P=0.006,8Foldchange)comparedtohealthycontrols.AsignificantcorrelationbetweentheexpressionlevelofRAD51andHER2/neu(P=0.024)aswellaspatientsage(P=0.03).Conclusion:ExpressionlevelofRAD51inlymphocytecellscanbeausefulbiologicalmarkerforevaluationofradiosensitivityinbreastcancer.

[学科分类] 基础医学